3-Dimensional reconstruction of fluorescent structures in tardigrades

3-Dimensional reconstruction of fluorescent structures in tardigrades

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Tardigrades are microscopic animals, thus brightfield microscopy is a well established method for tardigrade observation.Modern techniques in functional genetics like fluorescence in situ hybridisation or fluorescently labelled expression markers demand Course a pied - Femme - Vetements - Chandail - Zip high resolution fluorescence microscopy.Nevertheless tardigrades are still considered to be difficult objects for fluorescence techniques as they are covered by an opaque and diffracting cuticle.

We show a modern technique of structured 12VDC Camping Accessories light illumination that enables us to acquire thin optical sections and consequently to reconstruct 3-dimensional structures in tardigrades with a high spatial resolution in all 3 dimensions.This technique is evaluated on taxonomically valuable internal as well as external structures of eutardigrades: the bucco-pharyngeal apparatus and the claws.The 3-dimensional reconstructions allow the measurement of distances in all 3 dimensions.